性别:男
工作电话: 020-39943513
职称: 教授
电子邮件: lijfeng3@mail.sysu.edu.cn
导师类型: 博士生导师
学历: 博士
学科方向: 071001-植物学
0860-生物与医药
研究方向
（1）植物免疫信号转导（主要研究方向）
在电影《星际穿越》中，地球上的农作物先后被枯萎病团灭，只有玉米还在苦苦挣扎，人类终日只能吃土......现实中，病原菌引起的植物病害曾经对人类历史造成过深远影响。植物可以通过整合模式触发式免疫（PTI）和效应子触发式免疫（ETI），感知和抵御绝大多数病原菌。然而，少数开挂的病原菌能利用花样繁多的策略与植物大玩“躲猫猫”（逃避植物的免疫识别或阻断其免疫信号转导），以促进对植物的侵染。本课题组主要利用分子生物学、生物化学、细胞生物学、分子遗传学等手段探究植物感知病原菌以及免疫信号转导的分子机理。欢迎围观植物与病原菌这场不见硝烟的战争，学习安身立命的“三十六计”。
（2）植物靶向基因调控技术
基因缺失突变体的缺乏、基因功能冗余和基因缺失致死是植物基因功能研究中最主要的技术瓶颈。本课题组致力于建立和优化一套全方位的基因功能调控工具，可以实现高效的植物基因敲除、敲低与激活。
本课题组长期欢迎研究生与博士后青年人才加入！
个人经历和荣誉
1995年免试进入中山大学生物化学专业攻读本科，1999年免试进入中山大学生化与分子生物学专业攻读硕士，2002年起在中山大学生化与分子生物学专业攻读博士，于2005年获博士学位。2003-2004年在香港科技大学做访问学者（Visiting Scholar）。2005.11-2008.10在美国田纳西大学做博士后，2008.11-2014.8在美国哈佛医学院／麻省总医院任助理研究员（Research Fellow）。2014年9月起任中山大学生命科学学院教授、博士生导师。2018年起任生物科学与技术系系主任，2024年起任生命科学学院副院长，广东省植物逆境生物学重点实验室主任。
2014、2015、2021年先后获得国家海外高层次人才引进计划青年项目、国家优秀青年科学基金、国家杰出青年科学基金资助。
讲授课程
目前主要讲授《生命科学史》、《大学生物》、《现代生命科学进展》、《新生科研研讨课》。
学术成就
以通讯作者或第一作者（含共同）在Nature、Nature Biotechnology、Nature Plants（6篇）、Cell Host & Microbe（2篇）、Molecular Plant（2篇）、The Plant Cell（3篇）、Trends in Plant Science等SCI期刊发表论文58篇。
担任Plant Physiology、SCIENCE CHINA Life Sciences、aBIOTECH期刊的Editor，Cell、Nature Biotechnology、Nature Plants、Nature Communications、The Plant Cell、Molecular Plant 等期刊的审稿人以及德国教育科学部研究基金（BMBF）海外评审专家。
承担课题
- 中山大学百人计划科研启动项目（2015.1-2016.12）
- 国家海外高层次人才计划青年项目（2016.1-2018.12）
- 国家自然科学基金委优秀青年科学基金 No.31522006（2016.1-2018.12）
- 国家自然科学基金委面上项目 No.31570276（2016.1-2019.12）
- 国家自然科学基金委面上项目 No.31770295（2018.1-2021.12）
- 国家重大科技专项子课题 No.2019ZX08010003-001-009（2019.1-2020.12）
- 广州市科技计划重点项目 No.201904020041（2019.4-2022.3）
- 国家自然科学基金委面上项目 No.31970278（2020.1-2023.12）
- 国家重点研发计划子课题 No.2019YFA0906202（2020.1-2024.12）
- 国家自然科学基金委杰出青年科学基金No.32125004（2022.1-2026.12）
- 广东省基础与应用基础研究重大项目No.2023B0303000022 (2023.4-2028.4)
论文专著
通讯作者及第一作者论文（代表性论文）
（58）Li Z, Xiong X, Cao R, Li JF* (2026) Precision genetic manipulation: CRISPR toolkits reshape plant biology. Plant Physiology, accepted (Plant Physiology百年专刊特邀综述).
（57）Li C, Lin XX, Tian L, Sun W, Liu J, Liu Q, Du Y, Jiang X, Liang Z, Deng YZ, Au SWN, Wan JB, Li JF* (2026) N-glycosylation code diversifies paralogous chitinases to balance plant immunity and growth. Nature Plants, accepted.
（56）Wang FZ#, Bao Y#, Qiu J, Chen MX, Jiang H, Li Z, Xiong X, Jiang X, Kou Y*, Li JF* (2026) Cell-based CRISPRa screen identifies a papillae-specific receptor kinase mediating broad-spectrum fungal resistance in rice. Nature Plants, in press.
（55）Lin XX#, Li C#, Gong BQ, Liu JJ, Zhu LY, Tao K, Zhang D*, Li JF* (2026) A defense-inducible bidirectional promoter enables disease-resistance engineering in Arabidopsis without growth trade-offs. Plant Physiology, 201:1-13.
（54）Wang FZ，Bao Y，Yao X，Gong BQ, Xiong X, Wang J, Li Z, Li JF* （2026）AtLYK4 is the major chitin receptor in Arabidopsis with N-glycosylation and ligand-interacting residues orchestrating chitin perception. SCIENCE CHINA Life Sciences, 69: 638-650.
（53）Li Y#, Tao Y#, Yao X#, Cao R, Xiong X, Gong BQ, Li JF* (2025) SERK5 genetically compensates for the loss of BAK1 and BKK1 in regulating elf18-triggered immune signaling in Arabidopsis. Proc Natl Acad Sci USA, 122: e2513517122.
（52）Gong BQ#，Liu JJ#，Xu J，Cheng J，Li JF* （2025）Fungal oxalic acid inhibits CERK1 ectodomain deamidation to dampen chitin-triggered plant immunity. SCIENCE CHINA Life Sciences, 68: 3744-3755.
（51）Lin XX, Gong BQ, Wang FZ, Wan JB, Xiong X*, Li JF* (2025) Versatile applications of CRISPR-based programmable T-DNA integration in plants. Plant Biotechnology Journal, 23: 5950-5964.
（50）Sun W, Liu Q, Chen H, Xie X, Zhang Z, Zeng Y, Zhou J, Zhou X, Jiang X, Liang Z*, Li JF*, Deng Y* (2025) Rice phyllospheric Pantoea spp. suppress blast and bacterial blight diseases. Environmental Microbiome, 20: 137.
（49）Liu JJ#, Wen JX#, Li JF*, Wang FZ (2025) Nepenthes chitinase NkChit2b-1 confers broad-spectrum resistance to chitin-containing pathogens and insects in plants. Advanced Biotechnology, 3: 12.
（48） Zhang C#, Wu Y#, Liu J#, Song B, Yu Z, Li JF*, Yang C*, Lai J* (2025) SUMOylation controls peptide processing to generate damage-associated molecular patterns in Arabidopsis. Developmental Cell, 60: 696-705.e4.
（47） Li C#, Gong BQ#, Luo S, Wang T, Long R, Jiang X, Deng Y, Li JF* (2024) Engineering a conserved immune coreceptor into a primed state enhances fungal resistance in crops without growth penalty. Plant Physiology, 196: 2956-2972.
（46）Wang FZ#,*, Bao Y#, Li Z, Xiong X, Li JF* (2024) A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection of Cas9-free progeny in Arabidopsis. aBIOTECH, 5: 140-150.
（45）Ruan XM, Xiong X, Li JF* (2024) Identification and application of an exocarp-preferential promoter for genetic engineering of tomato fruit. Horticulture Research, 11: uhae035.
（44） Liu J#, Wang FZ#, Li C, Li Y, Li JF* (2023) Hidden prevalence of deletion-inversion bi-alleles in CRISPR-mediated deletions of tandemly arrayed genes in plants. Nature Communications，14： 6787.
（43）Xiong X#, Liu K#*, Li Z, Xia FN, Ruan XM, He X, Li JF* (2023) Split complementation of base editors to minimize off-target edits. Nature Plants, 9: 1832-1847.
（42） Wang FZ, Li JF* (2023) WeiTsing: a new face of Ca2+-permeable channels in plant immunity. Stress Biology, 3: 25.
（41）Li Y#, Xue J#, Wang FZ, Huang X, Gong BQ, Tao Y, Shen W, Tao K, Yao N, Xiao S, Zhou JM, Li JF* (2022) Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity. Nature Plants, 8: 802-816.
（40）Xiong X#, Li Z#*, Liang J, Liu K, Li C, Li JF* (2022) A cytosine base editor toolkit with varying activity windows and target scopes for versatile gene manipulation in plants. Nucleic Acids Research, 50: 3565-3580.
（39）Shen W, Zhang X, Liu J, Tao K, Li C, Xiao S, Zhang W, Li JF* (2022) Plant elicitor peptide signalling confers rice resistance to piercing-sucking insect herbivores and pathogens. Plant Biotechnology Journal, 20: 991-1005.
（38）Shen W, Cao S, Liu J, Zhang W, Chen J*, Li JF* (2021) Overexpression of an Osa-miR162a derivative in rice confers cross-kingdom RNA interference-mediated brown planthopper resistance without perturbing host development. International Journal of Molecular Sciences, 22: 12652.
（37）Yang Q, Guo J, Zeng H, Xu L, Xue J, Xiao S, Li JF* (2021) Receptor-like cytoplasmic kinase CDG1 negatively regulates Arabidopsis pattern-triggered immunity and is involved in AvrRpm1-induced RIN4 phosphorylation. The Plant Cell, 33: 1341-1360.
（36）Guo J, Gong BQ, Li JF* (2021) Arabidopsis lysin motif/F-box-containing protein InLYP1 fine-tunes glycine metabolism by degrading glycine decarboxylase GLDP2. The Plant Journal, 106: 394-408.
（35）Xiong X#, Liang J#, Li Z, Gong BQ, Li JF* (2021) Multiplex and optimization of dCas9-TV-mediated gene activation in plants. Journal of Integrative Plant Biology, 63: 634-645.
（34）Gong BQ#, Wang FZ#, Li JF* (2020) Hide-and-seek: Chitin-triggered plant immunity and fungal counterstrategies. Trends in Plant Science, 25: 805-816.
（33） Wang FZ#, Zhang N#, Guo Y#, Gong BQ, Li JF* (2020) Split Nano luciferase complementation for probing protein-protein interactions in plant cells. Journal of Integrative Plant Biology, 62: 1065-1079.
（32） Miao S, Liu J, Guo J, Li JF* (2020) Engineering plants to secrete affinity-tagged pathogen elicitors for deciphering immune receptor complex or inducing enhanced immunity. Journal of Integrative Plant Biology, 62: 761-776.
（31）Xue J#, Gong BQ#, Yao X#, Huang X, Li JF* (2020) BAK1-mediated phosphorylation of canonical G protein alpha during flagellin signaling in Arabidopsis. Journal of Integrative Plant Biology，62: 690-701 .
（30）Li Z, Xiong X, Li JF* (2020) The working dead: repurposing inactive CRISPR-associated nucleases as programmable transcriptional regulators in plants. aBIOTECH，1: 32-40.
（29） Gong BQ, Guo J, Zhang N, Yao X, Wang HB, Li JF* (2019) Cross-microbial protection via priming a conserved immune co-receptor through juxtamembrane phosphorylation in plants. Cell Host & Microbe， 26: 810-822.
（28）Shen W#, Liu J#, Li JF* (2019) Type-II metacaspases mediate the processing of plant elicitor peptides in Arabidopsis. Molecular Plant, 12: 1524-1533.
（27） Zhang D#, Zhang N#, Shen W*, Li JF* (2019) Engineered artificial microRNA precursors facilitate cloning and gene silencing in Arabidopsis and rice. International Journal of Molecular Sciences, 20: 5620.
（26）Li Z, Xiong X, Wang F, Liang J, Li JF* (2019) Gene disruption through base editing-induced messenger RNA missplicing in plants. New Phytologist, 222: 1139-1148.
（25）Xu L, Yao X, Zhang N, Gong BQ, Li JF* (2019) Dynamic G protein alpha signaling in Arabidopsis innate immunity. Biochemical and Biophysical Research Communications, 516: 1039-1045.
（24）Huang X#, Xue J#, Wang F*, Li JF* (2019) Enhanced protoplast assay by transfecting PCR-assembled gene expression cassettes with telomeric repeats and thiophosphate modifications. Analytical Biochemistry, 569: 39-45.
（23） Li Z#, Xiong X#, Li JF* (2018) New cytosine base editor for plant genome editing. Science China Life Sciences, 61: 1602-1603.
（22）Zhang N#, Zhang D#, Chen SL, Gong BQ, Guo Y, Xu L, Zhang XN, Li JF* (2018) Engineering artificial microRNAs for multiplex gene silencing and simplified transgenic screen. Plant Physiology，178: 989-1001.
（21）Liu J#, Liu B#, Chen S, Gong BQ, Chen L, Zhou Q, Xiong F, Wang M, Feng D, Li JF*, Wang HB*, Wang J* (2018) A tyrosine phosphorylation cycle regulates fungal activation of a plant receptor Ser/Thr kinase. Cell Host & Microbe，23: 241-253.
（20）Li Z#, Zhang D#, Xiong X, Yan B, Xie W, Sheen J, Li JF* (2017) A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants, 3: 930-936.
（19）Gong BQ, Xue J, Zhang N, Xu L, Yao X, Yang QJ, Yu Y, Wang HB, Zhang D, Li JF* (2017) Rice chitin receptor OsCEBiP is not a transmembrane protein but targets to plasma membrane via a GPI anchor. Molecular Plant, 10: 767-770.
（18）Zhang D, Li Z, Yan B and Li JF* (2016) A novel RNA-guided RNA-targeting CRISPR tool. Science China Life Sciences, 59:854-856.
（17）Zhang D and Li JF* (2016) DNA-guided genome editing tool. Science China Life Sciences, 59: 740-741.
（16） Zhang D, Li Z and Li JF* (2016) Targeted gene manipulation in plants using the CRISPR/Cas technology. Journal of Genetics and Genomics, 43: 251-262.
（15）Zhang D, Li Z and Li JF* (2015) Genome editing: New antiviral weapon for plants. Nature Plants, 1: 15146.
博士后期间
（14） Cheng ZY#, Li JF#, Niu Y, Zhang XC, Woody OZ, Xiong Y, Djonovic S, Millet Y, Bush J, McConkey BJ, Sheen J, Ausubel FM (2015) Pathogen-secreted proteases activate a novel plant immune pathway. Nature, 521: 213-216.
（13） Li JF*, Zhang D, Sheen J (2014) Epitope-tagged protein-based artificial microRNA screens for optimized gene silencing in plants. Nature Protocols, 9: 939-949.
（12） Li JF, Norville JE, Aach J, McCormack M, Zhang DD, Bush J, Church GM, Sheen J (2013) Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nature Biotechnology, 31: 688-691.
（11） Li JF*, Chung HS, Niu Y, Bush J and Sheen J (2013) Comprehensive artificial microRNA screens for effective gene silencing in plants. The Plant Cell, 25: 1507-1522.
（10）Liu B#, Li JF#, Ao Y#, Qu J, Li Z, Su J, Zhang Y, Liu J, Feng D, Qi K, He Y, Wang J and Wang HB (2012) Lysin motif-containing proteins LYP4 and LYP6 play dual roles in peptidoglycan and chitin perception in rice innate immunity. The Plant Cell, 24: 3406-3419.
（9） Li JF*, Bush J, Xiong Y, Li L and McCormack M (2011) High-throughput protein-protein interaction analysis in Arabidopsis mesophyll protoplasts by split firefly luciferase complementation. PLoS ONE, 6: e27364.
（8） Li JF*, Li L and Sheen J (2010) A rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology. Plant Methods, 6:1.
（7）Li JF*, Park E, von Arnim AG and Nebenführ A* (2009) The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species. Plant Methods, 5:6.
（6） Li JF* and Nebenführ A (2008) Interdependence of dimerization and organelle binding in myosin XI. The Plant Journal, 55:478-490.
（5）Li JF and Nebenführ A (2008) The tail that wags the dog: the globular tail domain defines the function of myosin V/XI. Traffic, 9:290-298.
（4）Li JF and Nebenführ A (2007) Organelle targeting of myosin XI is mediated by two globular tail subdomains with separate cargo binding sites. Journal of Biological Chemistry, 282:20593-20602.
博士期间
（3） Li JF, Qu LH and Li N (2005) Tyr152 plays a central role in catalysis of 1-aminocyclopropane-1-carboxylate synthase. Journal of Experimental Botany, 56:2203-2210.
（2） Li JF, Qi R, Qu LH, Mattoo AK and Li N (2005) Cleavages of the carboxyl-terminus of LeACS2, a tomato ACC synthase isomer, by a 64-kDa tomato metalloprotease produce a truncated but active enzyme. Journal of Integrative Plant Biology, 47:1352-1363.
（1） Li JF, Qu LH and Li N (2005) Isolation of a tomato protease that may be involved in proteolysis of 1-aminocyclopropane-1-carboxylate synthase. Journal of Integrative Plant Biology, 47:1220-1227.
第一作者或通讯作者论著
Li Z, Wang F, Li JF* (2019) Targeted transcriptional activation in plants using a potent dead Cas9-derived synthetic gene activator. Current Protocols in Molecular Biology, 127: e89.
Zhang N, Zhang D, Li JF* (2016) A simple protoplast-based method for screening potent artificial miRNA for maximal gene silencing in Arabidopsis. Current Protocols in Molecular Biology, 117: 26.9.1-26.9.9.
Li JF*, Zhang D and Sheen J (2015) Targeted plant genome editing via the CRISPR/Cas9 technology. Methods in Molecular Biology, 1284:239-255.
Li JF* and Zhang D (2014) Quantitative protein-protein interaction analysis by split firefly luciferase complementation in plant protoplasts. Current Protocols in Molecular Biology, 107: 20.9.1-20.9.8.
Li JF*, Zhang D and Sheen J (2014) Cas9-based genome editing in Arabidopsis and Tobacco. Methods in Enzymology, 546:459-472.
Li JF* and Sheen J (2012) DNA purification from multiple sources in plant research with homemade silica resins. Methods in Molecular Biology, 862:53-59.
Li JF and Nebenführ A (2010) FAST technique for Agrobacterium-mediated transient gene expression in seedlings of Arabidopsis and other plant species. Cold Spring Harbor Protocols, 5: pdb.prot5428.
