研究方向

主要聚焦于发展蛋白质组学前沿技术，包括空间蛋白质组学及翻译后修饰（如糖基化、磷酸化、泛素化）分析技术，以原位解析肿瘤微环境中细胞间信号转导的调控机制，并挖掘具有高特异性的生物标志物和潜在药物靶点。在研究前期，已成功开发多种创新蛋白质组技术，例如高效N-连糖蛋白富集技术（Nature, 2019）、空间糖蛋白组技术（Analytical Chemistry, 2019）以及基于免疫组化染色的组织空间蛋白质组技术（Analytica Chimica Acta, 2020）等。这些技术已成功应用于系统揭示胰腺癌中肿瘤细胞与基质细胞之间的信号转导机制，并挖掘出多个潜在创新药物靶点（Nature, 2025）。

 

个人经历

2007/09-2011/06，深圳大学，生物科学，学士

2011/09-2014/06，深圳大学，生物化学与分子生物学，硕士

2016/09-2020/11，香港浸会大学，分析化学，博士

2020.12-2025.05，南方科技大学，化学系，高级研究学者

2025.06-至今，中山大学生命科学学院副教授

 

承担课题

1. 中山大学引进人才计划，中山大学，2025/07-2028/07，主持，在研。

2. 深圳科创委科技重大专项，2024/01-2025/11，参与，在研。

3. 国家重点研发计划，2021/12 – 2026/11，参与，在研。

4. 国家自然科学基金委员会，面上项目，2022/01 – 2025/12，参与，在研。

 

论文专著（*通讯作者，#共同一作）

  1. Peiwu Huang#, Weina Gao#, Changying Fu#, Min Wang#, Yunguang Li#, Bizhu Chu, An He, Yuan Li, Xiaomei Deng, Yehan Zhang, Qian Kong, Jingxiong Yuan, Hebin Wang, Yu Shi*, Dong Gao*, Renyi Qin*, Tony Hunter, Ruijun Tian*. Clinical functional proteomics of intercellular signaling in pancreatic cancer. Nature, 2025, 637(8046):726-735.
  2. Peiwu Huang#, Weina Gao#, Changying Fu#, and Ruijun Tian*. Functional and Clinical Proteomic Exploration of Pancreatic Cancer. Molecular & Cellular Proteomics, 2023, 22(7):100575. 
  3. Min Tang#, Peiwu Huang#, Lize Wu, Piyu Zhou, Pengyun Gong, Xiang Liu, Qiushi Wei, Xinhang Hou, Hongke Hu, Ao Zhang, Chengpin Shen, Weina Gao, Ruijun Tian*, Chao Liu*. Comprehensive evaluation and optimization of the data-dependent LC-MS/MS workflow for deep proteome profiling. Analytical Chemistry, 2023, 95(20):7897-7905.
  4. Peiwu Huang, Qian Kong, Weina Gao, Bizhu Chu, Hua Li, Yiheng Mao, Zongwei Cai, Ruilian Xu, Ruijun Tian*. Spatial proteome profiling by immunohistochemistry-based laser capture microdissection and data-independent acquisition proteomics. Analytica Chimica Acta, 2020, 1127:140-148.
  5. Qian Kong#, Peiwu Huang#, Bizhu Chu, Mi Ke, Wendong Chen, Zhendong Zheng, Shanping Ji, Pengfei Li*, Zongwei Cai*, Ruijun Tian*. High-throughput and integrated chemical proteomic approach for profiling phosphotyrosine signaling complexes. Analytical Chemistry, 2020, 92(13):8933-8942.
  6. Peiwu Huang, Hongjie Li, Weina Gao, Zongwei Cai*, Ruijun Tian*. A Fully Integrated Spintip-based Approach for Sensitive and Quantitative Profiling of Region-resolved In Vivo Brain Glycoproteome. Analytical Chemistry, 2019, 91(14):9181-9189.
  7. Jianjun Liu#*, Peiwu Huang#, Yaqing He#, Wen-Xu Hong, Xiaohu Ren, Xifei Yang, Yanxia He, Wenjian Wang, Renli Zhang, Hong Yang, Zhiguang Zhao, Haiyan Huang, Long Chen, Dejian Zhao, Huixia Xian, Fang Yang, Dongli Ma, Linqing Yang, Yundong Yin, Li Zhou, Xiaozhen Chen, Jinquan Cheng*. Serum Amyloid A and Clusterin as Potential Predictive Biomarkers for Severe Hand, Foot and Mouth Disease by 2D-DIGE Proteomics Analysis.PLoS One, 2014, 9(9): e108816.
  8. Peiwu Huang#, Xiaohu Ren#, Zhijun Huang#, Xifei Yang, Wenxu Hong, Yanfang Zhang, Hang Zhang, Wei Liu, Haiyan Huang, Xinfeng Huang, Desheng Wu, Linqing Yang, Haiyan Tang, Li Zhou, Xuan Li, Jianjun Liu*. Serum proteomic analysis reveals potential serum biomarkers for occupational medicamentosa-like dermatitis caused by trichloroethylene. Toxicology Letters, 2014, 229(1): 101-110.
  9. Peiwu Huang#, Chao Liu#, Weina Gao, Bizhu Chu, Zongwei Cai, Ruijun Tian*. Synergistic optimization of LC and MS parameters on Orbitrap Tribrid mass spectrometer for high-efficient data dependent proteomics.Journal of Mass Spectrometry, 2021, e4653.
  10. 黄培武, 任晓虎, 洪文旭, 杨细飞, 张航, 李杰, 张艳芳, 刘建军*. 一例三氯乙烯药疹样皮炎患者不同发病时期血清蛋白质组分析. 中华预防医学杂志, 2014,48(5) : 420-421.
  11. 黄培武, 李绚, 任晓虎, 刘建军*. 三氯乙烯及其代谢物致癌性研究进展. 中华预防医学杂志, 2015,49(3) : 284-288.
  12. 黄培武，李绚，刘威，刘建军*. 三氯乙烯非致癌性毒性研究进展. 中华预防医学杂志, 2015,49(9) : 844-848.
  13. Yu Shi, Weina Gao, Nikki K. Lytle, Peiwu Huang, Xiao Yuan, Amanda M. Dann, Maya ridinger-Saison, Kathleen E. DelGiorno, Corina E. Antal, Gaoyang Liang, Annette R. Atkins, Galina erikson, Huaiyu Sun, Jill Meisenhelder, Elena terenziani, Gyunghwi Woo, Linjing Fang, Thom P. Santisakultarm, Uri Manor, ruilian Xu, Carlos R. Becerra, Erkut Borazanci, Daniel D. Von Hoff, Paul M. Grandgenett, Michael A. Hollingsworth, Mathias Leblanc, Sarah E. Umetsu, Eric A. Collisson, Miriam Scadeng, Andrew M. Lowy, Timothy R. Donahue, Tannishtha Reya, Michael Downes, Ronald M. Evans, Geoffrey M. Wahl, tony Pawson, Ruijun Tian* & Tony Hunter*. Targeting LIF-mediated paracrine interaction for pancreatic cancer therapy and monitoring. Nature, 2019, 569(7754):131-135.
  14. Lijun Yang, Jie Liu, Hua Li, Yilian Liu, An He, Peiwu Huang, Weina Gao, Hua Cao, Ruilian Xu*, Ruijun Tian*. A fully integrated sample preparation strategy for highly sensitive intact glycoproteomics. Analyst, 2022,147(5):794-798.
  15. Xueting Ye, Yun Yang, Jihao Zhou, Ling Xu, Long Wu, Peiwu Huang, Chun Feng, Peng Ke, An He, Guoqiang Li, Yuan Li, Yangqiu Li, Henry Lam, Xinyou Zhang*, Ruijun Tian*. Combinatory strategy using nanoscale proteomics and machine learning for T cell subtyping in peripheral blood of single multiple myeloma patients. Anal Chim Acta, 2021,1173:338672.
  16. Yihen Mao, Xi Wang, Peiwu Huang, Ruijun Tian*. Spatial proteomics for understanding the tissue microenvironment. Analyst, 2021, 146(12):3777-3798.
  17. Weina Gao, Qiaoyun Zhang, Yiran Su, Peiwu Huang, Xue Lu, Qinyue Gong, Wendong Chen, Ruilian Xu, Ruijun Tian*. Multiomic analysis of a dried single-drop plasma sample using an integrated mass spectrometry approach. Analyst, 2020, 145(20):6441-6446.
  18. Xue Lu#, Zhikun Wang#, Yan Gao, Wendong Chen, Lingjue Wang, Peiwu Huang, Weina Gao, Mi Ke, An He, Ruijun Tian*. AutoProteome chip system for fully automated and integrated proteomics sample preparation and peptide fractionation. Analytical Chemistry, 2020, 92(13):8893-8900.
  19. Nuanyuan Luo, Qunqun Chang, Xiaohu Ren, Peiwu Huang, Wei Liu, Li Zhou, Yungang Liu, Jianjun Liu*. Trichloroethylene injures rat liver and elevates the level of peroxisomal bifunctional enzyme (Ehhadh). Molecular & Cellular Toxicology, 2020, 16:331-345.
  20. Weina Gao, Hongjie, Liping Liu, Peiwu Huang, Zhikun Wang, Wendong Chen, Mingliang Ye, Xiaofang Yu, Ruijun Tian*. An integrated strategy for high-sensitive and multi-level glycoproteome analysis from low micrograms of protein samples. Journal of Chromatography A, 2019, 1600:46-54.
  21. Xue Lu, Lin Lin, Wenbin Zhou, Wendong Chen, Jun Tang, Xiujie Sun, Peiwu Huang, Ruijun Tian*. Mixed-mode ion exchange-based integrated proteomics technology for fast and deep plasma proteome profiling. Journal of Chromatography A, 2018, 1564:76-84.
  22. Subash Adhikari, Lan Chen, Peiwu Huang, Ruijun Tian*. Proteomic Analysis of Secreted Proteins from Cell Microenvironment. Methods in Molecular Biology, 2017, 1662:45-58.
  23. Peipei Song, Peiwu Huang, TJ Huang, Hua Li, Wendong Chen, Lin Lin, Shun Feng*, and Ruijun Tian *. Facile synthesis of carboxymethyl-β-cyclodextrin conjugated magnetic nanoparticles for selective enrichment of glycopeptides. Rapid Communications in Mass Spectrometry, 2016, Suppl 1:190-195.
  24. Xuan Li, Xiyi Li, Zhiliang Zhu, Peiwu Huang, Zhixiong Zhuang, Jianjun Liu, Wei Gao, Yinpin Liu, Haiyan Huang*. Poly(ADP-Ribose) Glycohydrolase (PARG) Silencing Suppresses Benzo(a)pyrene Induced Cell Transformation. PLoS One, 2016, 11(3):e0151172.
  25. Xiaohu Ren, Jie Li, Bo Xia, Wei Liu, Xifei Yang, Wen-Xu Hong, Peiwu Huang, Yong Wang, Shuiming Li, Fei Zou, Jianjun Liu*. Phosphoproteomic analyses of L-02 liver cells exposed to trichloroethylene. Toxicology Mechanisms and Methods, 2015, 25(6):459-466.
  26. Wei Liu, Wen-Xu Hong, Yanfang Zhang, Peiwu Huang, Xifei Yang, Xiaohu Ren, Haiyan Huang, Jianjun Liu*. Proteomic profiling of occupational medicamentosa-like dermatitis induced by trichloroethylene in serum based on MALDI-TOF MS. Clinical and Experimental Medicine, 2015, 15(4):519-526.
  27. Xiaohu Ren, Xifei Yang, Wen-xu Hong, Peiwu Huang, Yong Wang, Wei Liu, Jinbo Ye, Haiyan Huang, Xinfeng Huang, Liming Shen, Linqing Yang, Zhixiong Zhuang, Jianjun Liu*. Identification of the proteins related to SET-mediated hepatic cytotoxicity of trichloroethylene by proteomic analysis, Toxicology Letters, 2014, 227(1): 12-19.
  28. Xiaojing Sui, Xiaohu Ren, Peiwu Huang, Shuiming Li, Ma Quan, Ming Ying, Jiazuan Ni, Jianjun Liu *, Xifei Yang. Proteomic analysis of serum proteins in triple transgenic Alzheimer's disease mice: implications for identifying biomarkers for use to screen potential candidate therapeutic drugs for early Alzheimer's disease, Journal of Alzheimer's disease, 2014, 40(3): 575-586.
  29. Wen-Xu Hong, Wei Liu, Yanfang Zhang, Peiwu Huang, Xifei Yang, Xiaohu Ren, Jinbo Ye, Haiyan Huang, Haiyan Tang, Guifeng Zhou, Xinfeng Huang, Zhixiong Zhuang, Jianjun Liu*. Identification of serum biomarkers for occupational medicamentosa-like dermatitis induced by trichloroethylene using mass spectrometry, Toxicology and Applied Pharmacology, 2013, 273(1): 121-129.
  30. 高伟娜, 黄培武, 田瑞军*. 基于质谱技术的糖蛋白质组学的分析方法及其在癌症研究中的应用. 生命的化学, 2017,37(1):121-131.
  31. 杨细飞, 黄培武, 汤瑞华, 任晓虎, 洪文旭, 刘建军, 何雅青, 程锦泉*. 肠道病毒71型致轻症手足口病患儿血清蛋白质组分析. 中国热带医学, 2014,14 (4): 387-391.
  32. 张航, 胡俊杰, 汤瑞华, 叶金波, 任晓虎, 胡韬, 黄培武, 杨细飞, 黄海燕, 刘建军*. 高效液相色谱-串联质谱检测基因组DNA甲基化方法的建立. 生物技术通讯. 2014,25(04): 537-541.
  33. 叶金波, 刘威, 周桂凤, 任晓虎, 黄培武, 洪文旭, 黄海燕, 刘建军*. 职业性三氯乙烯药疹样皮炎诊断模型的建立. 中华预防医学杂志. 2013,(02): 151-154.